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ACS Nano 15, 1466214671

JIMD Rep 2017;32:117-24

The membranes were then washed several times with water and TBS (PBS, 0.05% Tween-20), a 1 :5000 dilution of horseradish-peroxidase conjugated goat anti-rabbit antiserum was added, and the membranes further incubated for 1 h at room temperature

This includes aspects like texture, clarity, and overall freshness, which are often associated with well-maintained skin