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Quantification of the time course measurements of the fluorescence ratio (F400/488) for HeLa cells with lentivirus mediated LUC, Lb NOX, Ec STH, mito Ec STH expression under Dox control transiently expressing iNAP1 in the basal medium followed by replacement with basal medium without glucose but with 10 mM pyruvate ( b ), after addition of G6PDi ( c ), after addition of 6-AN ( d ), or after addition of diamide ( e )

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Most people start at 1mg and increase to 2mg after week 12