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Analytes (M-CuP or D-CuP) were diluted in PBST buffer (10 mM phosphate-buffered saline, 0.05% Tween-20, pH 7.4) and injected at increasing concentrations at a flow rate of 30 L/min, with 120 s association and 180 s dissociation phases

93 Wnt/-catenin signaling pathway The Wnt signaling pathway is categorized into the canonical (-catenin dependent) and noncanonical (-catenin independent) pathways

After exposure to non-lethal ischemia for 1 h, these mice were allowed to recover for another 24 h before sacrifice (Supplementary Fig