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The resulting oxidized form of GSH (GSSG), characterized by a disulfide bond between two molecules of GSH is efficiently reduced back to GSH by the NADPH-dependent catalysis of the flavoenzyme GSH reductase

In the glyoxalase system, the rate-limiting enzyme is glyoxalase 1 (Glo-1, lactoylglutathione lyase)

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The association between coffee intake and the urinary concentrations of 8-hydroxydeoxyguanosine (8-OHdG), a biomarker of systemic oxidative damage and DNA repair, was analyzed: by examining more than 200 female subjects, the data showed that the urinary concentrations of 8-OHdG tended to decrease in women with a daily intake of 23 cups of coffee

Human evidence - Phase 2 type 2 diabetes (Rosenstock et al., Lancet 2023) Adults with type 2 diabetes, 36 weeks