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REM sleep is characterized by a rapid eye movement

The observed injuries in the lungs, heart, kidneys, and brain share a common mechanistic hallmark: glycocalyx shedding, increased vascular permeability, neutrophil adhesion, and oxidative stress [5, 17, 21, 104]

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When fully dissociated, cells were centrifuged (230 g , 3 min) and the cell pellet resuspended and diluted to approximately 2.5E06 cells/mL in Recovery TM Cell Culture Freezing Medium (Gibco, #12648-010)