Volume was infused through a syringeperfusion pump system (Argus 600, Argus Medical A6, Heimberg, Switzerland) (1 ml/10 s) in 10 cm colon segment with colitis and with anastomosis between 5 cm orally and 5 cm aborally, and corresponding 10 cm colon segment with colitis but without anastomosis
NF-B plays a critical role in the initiation and perpetuation of inflammation
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Subsequently, 180 l fresh culture medium and 20 l of samples (0.05858000 M in sterile water) were added per well and incubated for 24, 48 and 72 hours
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