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However, prior studies generally evaluate allosteric IDH1 inhibitors in cell-free systems under conditions that do not represent the tumor microenvironment ( 30, 33, 34 )

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Protein and lipid pellets were discarded, while 10 L injections of supernatants were analyzed via a Vanquish UHPLC system (Thermo Fisher Scientific) coupled to a Q Exactive mass spectrometer (Thermo Fisher Scientific) utilizing an untargeted acquisition as previously reported 18