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The proliferation of progenitor cells including ASCs, stability of DNA, and morphological integrity are fundamental factors that impact ASCs stemness, pro-regenerative ability, and finally clinical usefulness [51,52,53]

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The maintenance frequency approach represents hybrid strategy where initial intensive treatment (daily application for 3-6 months) establishes improvements, then reduces to maintenance schedule (2-3 times weekly or every other day) rather than complete discontinuation

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