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The obtained crude liposomes were subjected to five freezethaw cycles in liquid nitrogen and then extruded six times (twelve passes) through a polycarbonate membrane with 100-nm pore diameter using a hand-held Avanti mini extruder (Avanti Polar Lipids Inc

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All cultures were maintained in the presence of 1% penicillin- streptomycin

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Pack Size: Single vial (1200mg)