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After treatment, cells were fixed with 4% paraformaldehyde (PFA) for 15 min before permeabilization with 0.1% saponin in blocking buffer (1% fatty acid free BSA, 20 mM glycine in PBS) for 15 min at room temperature

Analyses of sequence evolution across the coronavirus family through evolutionary timescales and throughout the pandemic can help to identify regions that are conserved and, if immunogenic, amenable to eliciting broadly reactive immunity , especially in the context of selection pressure from increasing levels of vaccine-induced and infection-induced immunity

Silvano Junior Santini, University of L'Aquila, Italy Updates Copyright 2023 Santacroce, Gentile, Soriano, Novelli, Lenti and Di Sabatino

Impact of chitosan composites and chitosan nanoparticle composites on various drug delivery systems: a review