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Composition Active Agent: Benzyl Alcohol 0.9% (bacteriostatic preservative) Base: Sterile Water Volume: 10ml or 30ml per vial (select above) pH: 4.57.0 Container: Sealed glass vial with rubber septum and aluminium crimp cap Manufactured in: Boecillo (Valladolid), Spain ISO 13485:2016 + GMP + ISO 9001:2016 certified facility Intended use: Research Use Only (RUO) not for clinical use In-use period: Up to 28 days after first puncture with aseptic technique Storage Store unopened vials at room temperature (15-25C)

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Single oocytes were lysed in proteinase K-containing buffer and processed at 55C for 2 hours, followed by enzyme inactivation at 95C