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Transfections of siRNA were carried out using Lipofectamine RNAi-Max (Life Technologies) following the manufacturers protocol

The study authors found that intravenous injection of 42mg to 1260mg of TB4 was safe in healthy adults, with no dose limiting toxicities or serious adverse events reported [26]

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For identification, lipids with 3 and 4 stars were automatically approved, and searched for matching adducts and in-source fragments