The triple combination upregulated UBE2C, TTC5, ELOVL5 and NDUFS8
Phospho-peptide enrichment was performed using TiO 2 (Titansphere Phos-TiO Bulk 10 um, GL Science), ratio of beads to sample 10:1, mixed for 30 minutes at room temperature in 80% acetonitrile, 6% trifluoroacetic acid, 5 mM monopotassium phosphate, 20 mg/mL 2.5-dihydroxybenzoic acid
Historical Schilling test results showing impaired B12 absorption correctable with intrinsic factor also confirm the diagnosis
Because a B12 injection bypasses all potential barriers to absorption, it can help reverse deficiency symptoms relatively fast
When B12 levels are too low, the nerves can become damaged, leading to sensations like numbness, tingling, or a pins and needles feeling in the hands, feet, or legs