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BALF was centrifuged to collect supernatants for cytokine and total protein content analysis, while cell pellets were used for differential cell counts via cytospin centrifuge and Hema-3 staining
Phytosterols like -sitosterol primarily act by interfering with cholesterol-dependent cell functions and oncogenic signaling
Insulin, periodic acid-Schiff, pethenamine silver, and Masson's trichrome staining were also completed to clearly visualise pancreatic beta cells, blood vessels, and collagen, respectively
[10] Gastrointestinal disturbances-including nausea, loose stools, and a transient fishy body-odor attributable to trimethylamine production from excessive carnitine and choline metabolism-occur in a minority of recipients and are usually self-limiting within 24 to 48 hours after injection
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