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Furthermore, translational challenges such as tissue targeting (e.g., bloodbrain barrier penetration), metabolic stability (e.g., optimization of CoQ analogs), and the need for predictive biomarkers underscore the importance of continued mechanistic studies to refine therapeutic strategies exploiting these emerging ferroptosis defense nodes

Total cysteine and GSH were analyzed using the SBD-F sample derivatization method (22)

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In the genetic engineering strategy, researchers have induced tumorigenesis by targeting and integrating oncogenic gene expression vectors or using targeted knockdown of tumor suppressor genes mediated by the CRISPR/Cas9 system ( Xenotransplantation serves as the primary methodology for developing zebrafish breast cancer models ( Figure 1 ), involving engraftment of foreign tissues across species barriers