Cells were collected via dissociation by TrypLE Express and pelleted by centrifugation (300 x g , 3 min, room temperature in 15-ml conical tubes, followed by 21,000 x g , 2 min, room temperature in microcentrifuge tubes) and stored at -80C until further analysis
"Serum N(1)-Methylnicotinamide Is Associated With Obesity and Diabetes in Chinese." Journal of Clinical Endocrinology & Metabolism
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Their mechanism involves enhancing mitophagy, suppressing NLRP3 inflammasome activation in hepatocytes, and modulating key metabolic regulators
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