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[69] developed a novel HBsAg and HBcAg combined vaccine, which was evaluated for its immunogenicity and therapeutic effects in HBV transgenic mice using different adjuvants

W.WangJ.AbmanS

Then the total lysates were run on polyacrylamide gels, proteins were blotted on PVDF-membranes and the membranes were blocked in blocking buffer for 1 h

Tufekci KU, Ercan I, Isci KB, Olcum M, Tastan B, Gonul CP, et al

Buffer systems comprise mixtures of appropriate amounts of an acid such as phosphoric, succinic, or preferably citric acid, and a base, in particular sodium hydroxide