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The nuclei were bound to activated Concanavalin beads and incubated with the primary antibody (1:100, DO-1, Santa Cruz Biotechnology, Dallas, United States) or isotype control overnight at 4 C

Although probiotic supplementation shows promising potential in improving thyroid function in thyroid diseases, further human studies on the effects of probiotics as adjuvant therapy for thyroid diseases are required ( Table 1 )

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