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The reaction was quenched by the addition of 5 Laemmli and 400 M mmPEG and incubation for 15 min on ice

Miller MA, Zachary JF (2017) Mechanisms and morphology of cellular injury, adaptation, and death

Tumours and tissues were snap-frozen on dry ice and stored at 80 C or placed in 10% neutral buffered formalin (Fisher Scientific, 22-110-761) for 24 h and then stored in 100% methanol (Fisher Scientific, A412P-4)

Statistical analysis All the statistical analyses were performed with GraphPad Prism 10.0 software (GraphPad Software, San Diego, CA)

This action helps protect DNA, proteins, cell membranes, and mitochondria from damage