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Cell debris was removed by centrifugation at 4400 g at 4 C for 30 min and the clarified cell lysate was used for protein purification

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Severe Hypokalemia: A Critical Drop in Potassium Levels One of the most serious complications of B12 replacement therapy in severe megaloblastic anemia (a type of anemia where blood cells are abnormally large) is the development of severe hypokalemia (dangerously low potassium levels in the blood)

[DOI] [PMC free article] [PubMed] [Google Scholar] 129.Li X, Meng Y, Plotnikoff NP, Youkilis G, Griffin N, Wang E, et al

Mol Cell Biol 1999