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We show (i) that PfGrx-catalyzed redox reactions outcompete uncatalyzed reactions between roGFP2 and GSH or GSSG by several orders of magnitude, (ii) that the PfGrx-catalyzed reduction and oxidation of roGFP2 occur via a mono- and not a dithiol mechanism, and (iii) that the reduction of roGFP2(S 2 ) as a model non-glutathione disulfide substrate most likely involves a rate-limiting glutathionylation in a ternary complex with a GSH molecule that is activated at the 1st glutathione-interaction site of PfGrx
Alongside carbohydrates and fats, proteins are among the most important macronutrients
Research documents effects on endothelial integrity and vascular inflammatory responses
186 This suggests that targeting USP20 may mitigate chemotherapy resistance and promote ferroptosis in HCC patients, and explains why DNA damage therapy often leads to treatment resistance
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