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The dried samples were reconstituted using 200 HPLC mobile phase (10% v/v ACN in water), vortexed briefly, and subjected to HPLC separation and analysis

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Another potential limitation of this study is that we only used one assay kit to quantify levels of GSH in our plasma samples

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EE focuses on the fraction of drug encapsulated versus total input, while loading capacity measures how much drug is contained relative to the total liposome or lipid mass