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The supernatants were transferred to clean tubes and samples stored at 4 C

The thing is, that questions is answered with another question: how do you know if there is room for improvement

Rather than indiscriminately blocking macrophage activity (which would impair the early inflammatory debridement phase of wound healing), GHK-Cu research documents a shift in macrophage phenotype from pro-inflammatory M1 toward the anti-inflammatory, pro-healing M2 profile at later stages of the inflammatory response

The mice were housed in cages placed in a quiet and temperature-humidity controlled room (22C1-2C) with a 12 hr light-dark cycle

Staheli, D.O