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used time-resolved fluorescence anisotropy to analyze the 8 kDa protein kinase inhibitor (PKI alpha) of cAMP-dependent protein kinase (cAPK) free in solution and bound to cAPK (Hauer et al., 1999)

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PLoS One 11(11):e0165595 Yu Q, Stamenkovic I (2000) Cell surface-localized matrix metalloproteinase-9 proteolytically activates TGF-beta and promotes tumor invasion and angiogenesis