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While our qPCR analysis relied on a single reference gene (GAPDH) that showed stable expression, future studies could benefit from incorporating multiple reference genes as recommended by the MIQE guidelines [51]

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The RNA was then treated with RapidOut DNA Removal Kit (K2981, Thermo) according to the user guide to remove trace genomic DNA residue

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Choosing an injection site The most common subcutaneous injection sites for retatrutide are: Abdomen: 2 inches away from the navel, left or right side