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In summary, the overwhelming production of ROS during MIRI, coupled with the depletion, inactivation, and dysregulation of the intrinsic antioxidant defense mechanisms and their regulatory pathways, leads to a state of severe redox imbalance

This concentration is the standard formulation for multi-dose research-grade reconstitution solutions

Cells were seeded at a density of 510 3 /well in 200 L of medium into 96-well plates (BIOFIL, Guangzhou, China), and treated with Erastin or ferrostatin-1 for the indicated time