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The resultant files were processed with El-Maven (Elucidata) alongside the Kyoto Encyclopedia of Genes and Genomes (KEGG) 20,21,22 database for metabolite assignment and manual peak integration as previously described using intact mass, isotopic patterns, and an in-house standard library 23

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Contaminants or incorrect amino acid sequences in either peptide can lead to inaccurate or misleading data

The effects of melatonin daily supplementation to aged rats on the ability to withstand cold, thermoregulation and body weight