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For a deeper explanation, see How Long Do Reconstituted Peptides Last When to Use Sterile Water Sterile water is typically preferred in controlled, single-use scenarios

After blocking with 5% BSA-TBST (Sigma), the membranes were incubated with the following primary antibodies from Abcam at 4C overnight: ER (1:1,000, ab3575), ER (1:1,000, ab3576), Ggt1 (1:1,000, ab175384), and Anpep (1:1,000, ab108310)

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this generally results from genetic variability in the enzymes needed to produce and/or regenerate it 2

ITF is a small peptide found abundantly throughout the gut and known to concentrate at sites of injury, such as in proximity to peptic ulcers [113]