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DNA was extracted from the fecal samples using the DNeasy PowerSoil Kit (Qiagen, Hilden, Germany)

Mandayam 2006)

some peptides dissolve slowly Gently warm the vial to 37 C in a water bath (do not exceed 37 C) Add bacteriostatic water in small increments (e.g., 0.5 mL at a time) Some peptides (e.g., semaglutide, retatrutide) have fatty-diacid chains that reduce solubility

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