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Cell debris was removed by centrifugation at 4400 g at 4 C for 30 min and the clarified cell lysate was used for protein purification

Studies have shown that X-ray irradiation reduces KGN cells viability, alters mitochondrial morphology, induces intracellular iron accumulation, and triggers lipid peroxidation, ultimately leading to ferroptosis (Zhao et al., 2023)

Distributes systemically

Niacinamide does not cause the dramatic flush of niacin, so titration to mitigate flushing is usually not necessary

X-ray photoelectron spectroscopy was conducted on a Thermo Scientific TM K-Alpha TM+ spectrometer equipped with a monochromatic Al K X-ray source (1486.6 eV) operating at 100 W