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This is important in the case of analysis of biological samples, where as a rule large excesses of reducing agents are used to break all disulfide bonds

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Sample Derivatisation Sample derivatisation was carried out according to a modification of method of Kander et al

Resistance is futile: targeting mitochondrial energetics and metabolism to overcome drug resistance in cancer treatment

Sodium phosphate (50 mM pH 7.4), EDTA (1 mM), GSH (2 mM), NADPH (0.25 mM), glutathione reductase (1 U) and samples (2.5 M) were mixed in a cuvette at 37C