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5 days/week) Frequency: Once daily, 5 days per week , progressing from 1.0 mg to 1.5 mg and then 2.0 mg as shown above

The kits were purchased from Nanjing Jianjian Bioengineering Institute, and the activities of alkaline phosphatase ALP, acid phosphatase ACP and superoxide dismutase SOD were determined according to the instructions

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For protein immunoprecipitation experiments, PC-3 cells were obtained in culture and transfected with FLAG, FLAG-FBI-1 in the cells, after which the cell samples were collected for fragmentation [49,50]