In a study on the fungal pathogen Histoplasma capsulatum , macrophages treated with the glucose analog 2-deoxy-D-glucose (2-DG) showed reduced exogenous zinc import, resulting in zinc starvation, which led to fungal death
Further analysis by Western blot showed that rhGPx3 mutant with excess amino acid could be recognized by the monoclonal antibody against the hexahistidine tag, but not in the case of the protein treated with factor Xa (Figure 1a and 1b)
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