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Carotenoid accumulation also peaked at intermediate Mg levels, with the highest concentrations found at 36.9 mg/L

In vitro transcription to prepare biotinylated PRKAA1 5UTR and scrambled RNA probes PRKAA1 5UTR and scrambled control RNA probes were synthesized using BamHI-linearized pUC57-T7 templates in an 80-l reaction buffer containing 5 transcription buffer (Roche, RPOLT7-RO), 8 l of 10 Dig labeling mixture (Merck, 11277073910), 8 l of 0.1 M DTT (Sigma, D9163

Jan-Feb 2021;11(1):91-100

The log-rank test did not demonstrate differences among the two curves ( p = 0.654) (Supplementary Figure S2)