A typical reaction contained 25 M enzyme, 5 mM ()- t -ES or other dicarboxylic acid, 2.5 mM amino acid, 10 mM ATP and 10 mM MgCl 2 in 100 l of 50 mM sodium phosphate buffer (pH 8.0)
Williams TL, Verdon G, Kuc RE et al (2024) Structural and functional determination of peptide versus small molecule ligand binding at the apelin receptor
doi: 10.1016/S0741-8329(02)00229-X, PMID: [DOI] [PubMed] [Google Scholar] 69.Videla LA, Rodrigo R, Orellana M, Fernandez V, Tapia G, Quiones L, et al
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