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After this period, the transfection media were discarded and replaced with fresh DMEM medium supplemented with 0.5% FBS and 1% antibioticantimycotic solution

Cytotechnology 53 , 8193 (2007)

Cellular senescence is characterized by a stable arrest in the cell cycle and encompasses a range of cellular changes that culminate in the development of a complex, pro-inflammatory secretory profile known as the senescence-associated secretory phenotype (SASP), which plays a critical role in fibrosis and tumors by affecting numerous processes, including invasion and therapy resistance 10

Materials and methods Study design and procedure Data were collected as part of a large multisite study of pediatric concussion, Advancing Concussion Assessment in Pediatrics (A-CAP))

The incubation period of coronavirus disease 2019 (COVID-19) from publicly reported confirmed cases: estimation and application