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These substances exhibit mechanisms akin to probiotics by having the capacity to modify intestinal pH and suppress the proliferation of pathogenic microorganisms, thereby contributing to intestinal homeostasis

Western blot analysis Protein lysates were obtained by homogenizing tissues or cell pellets in sample buffer containing 62.5 mM Tris-HCl (pH 6.8), 4% SDS, 200 mM dithiothreitol (DTT), 10% glycerol, and 0.001% bromophenol blue using a tissue to buffer ratio of 1:10 (w/v), followed by boiling

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171 In line with this, ROS-deficient Ncf1** mice which was characterized by the inability to produce ROS and represent mice model of CGD, showed a reduction in NETs aggregation and an increase in inflammatory mediators during the stimulation by MSU crystals when compared to air pouches of wild-type mice