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Quantitative PCR To analyze mRNA expression, total RNA was collected from cells using the PureLink RNA Mini Kit (Thermo Fisher Scientific), and 1 g was reverse-transcribed using the ProtoScript II First Strand cDNA Synthesis Kit with oligo(dT) primers and murine RNase inhibitor (New England Biolabs)

Western blot analysis revealed the hepatic LDLR expression levels

WCBs offer many advantages, including the low cost of growing small quantities of bacterial culture and access to a range of configurable and orthogonal output signal detection modalities, including luminescence, fluorescence and colorimetric approaches

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