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For the untargeted experiment, proteins were digested with 0.7 g of trypsin (sequencing grade, Promega, Madison, WI) and 0.7 g of Lys-C (New England Biolabs) for 1 h at 37 C

The feedback inhibition of IGF-1 on further GH secretion stayed intact, preventing the GH excess that drives insulin resistance with direct injection

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This is one of a series of commentaries under the banner 50 years forward, giving personal opinions on future perspectives in diabetes, to celebrate the 50th anniversary of Diabetologia (19652015)

The Spirulina methylation also helps in facilitation of the heavy metals biosorption which shows higher uptake of the contaminants (Aravind et al