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69 compared GSH blood concentrations in healthy young and healthy elderly subjects and found that the reference group of 20- to 39-year-old subjects (n = 40) had a GSH concentration 17% higher on average than the 60- to 79-year-old cohort of 60 subjects (mean SD, 547 53.5 g/10 10 vs

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Cell lines and cell culture Human embryonic kidney 293 (HEK293, #CRL-1573) cells were obtained from ATCC and were cultured in DMEM/F-12 (Dulbeccos Modified Eagle Medium/Nutrient Mixture F-12, Gibco #11330-032) medium with 10% fetal bovine serum (FBS, Sigma-Aldrich #F2442) and 1x penicillin streptomycin (Corning #30-002 CL) at 37 C in a 5% CO2 incubator

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