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Mouse livers were first perfused with Krebs-Ringer buffer containing 100 M EGTA (MilliporeSigma) to chelate calcium, followed by enzymatic digestion using 60 mg of type I collagenase (Thermo Fisher Scientific) dissolved in 50 mL of DMEM/F12

The PV type 1 Sabin, which is BSO-resistant, contains mutations in all of the capsid proteins but it is not known which one is responsible for the BSOr phenotype

doi: 10.1016/S0021-9258(18)43421-7