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In each case, the newly produced GSH was quantified using the Glutathione Assay Kit # 703002 (Cayman Chemical) described as previously
Individually or cumulatively, age-related modifications destabilize crystallins, leading to their partial unfolding and conversion to large protein aggregates that scatter light and cause the lens opacification characteristic of ARN cataract [117]
From these, we selected for estimation parameters that were not available from literature and that are most directly associated with the dynamics of the GSH and GSSG concentrations ([GSH] and [GSSG], respectively), for which we had available experimental data
