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Description

RT significantly increased myofibrillar protein synthesis by 47%, enlarged type I and IIa fiber cross-sectional area by 16 and 28%, raised total lean mass by 2%, and reduced frailty criteria by 64%, improving strength and physical function

Spheroid formation SF8628 cells were detached from the cell culture flask by treating with 0.25% trypsin-EDTA (Gibco, Thermofisher Scientific, Reinach, Switzerland) for 3 min at room temperature and were resuspended in DMEM at a density of 5000 cells ml 1