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The filtered solution was naturally cooled and slowly stirred at room temperature, then stored overnight at 4 C

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Yes, coffee enemas are particularly dangerous for people with inflammatory bowel disease (Crohn's disease or ulcerative colitis), diverticulitis, hemorrhoids, recent bowel surgery, heart conditions, kidney disease, or electrolyte imbalances

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Chang JY, Zhang W, Komaki R, Choi NC, Chan S, Gomez D, O'Reilly M, Jeter M, Gillin M, Zhu X, Zhang X, Mohan R, Swisher SG , Hahn S, Cox JD