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Indeed, erectile dysfunction (ED) can be exacerbated by problems with the prostate

The cells were lysed by ultrasound for 20 min at 4 C, followed by centrifugation at 12,000 rpm for 15 min to collect the supernatant

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Then, tissue samples were cut and weighed into 100 mg sections and mixed with 400 L of methanol in tubes containing 1.0-mm zirconia beads (BioSpec Products, USA) and homogenized using a BeadBug bead homogenizer (Benchmark Scientific, USA) at 4000 rpm for 2 min

Dose adjustment, protocol modification, or reevaluation of injury severity and type may be appropriate