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20 mL EDTA 0.5 M, 80 mL SDS 10%, and 2 mg proteinase K were added to each reaction and incubated overnight at 65 C to digest proteins

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In response to herbivorous insect attack or wounding at local leaves, wound signaling molecules are released at the damaged plant cells, and may bind corresponding receptors to trigger propagation of slow wave potentials (SWPs) 6,7,8,9 , initiate transmission of cytoplasmic calcium ([Ca 2+ ] cyt ) 10,11,12,13,14,15 , and consequently activate biosynthesis of defensive phytohormone jasmonic acid (JA), thereby mediating expression of defense-responsive genes for systemic defense responses in plants 7,8,11,12,16,17,18

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Am J Geriatr Psychiatry 2004