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Finally, we evaluated only the EZH2 and H3K27me3 proteins and not EZH2 and H3K27me3 mRNAs in cells exposed to cigarette smoke, since as previously showed 17 , this methodological approach seems to be adequate to define the epigenetic modifications of DAB2IP gene, by the action of methyl transferase EZH2 on H3K27 trimethylation in lysine 27

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A 4-second draw counts as one puff but uses roughly twice the e-liquid of a 2-second draw