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Gene expression was measured on the 7500 Fast Real-Time qPCR System (Thermo Fisher) using SYBR Green (New England Biolabs) and primers listed in Supplementary Table S1

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Sepiapterin, a precursor in BH4 biosynthesis, can inhibit age-related ED and decrease OS by preventing eNOS uncoupling [90]

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Studies are examining how Cag influences glucagon suppression, glucose absorption, and overall glycaemic control in metabolic research models

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