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Blood samples were collected from the retro-orbital plexus puncture (under general anesthesia) of the mice using a sterile hematocrit capillary tube before (pre-immune, 23 days prior) and after each antigen/protein booster immunization, ideally 2 days preceding each booster dose, as outlined in Figure 1 (for Pf CSP) and Supplementary Figure S3B (for Pb SLTRiP)

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