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The combination of the N-terminal amine, the histidine imidazole, and the intervening amide nitrogen is a recurrent high-affinity copper-binding motif in peptide biochemistry it appears in human serum albumin's N-terminal copper-binding site, in various other copper-binding peptides, and in synthetic copper-binding peptide design

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Infected cells were then incubated in a humidified CEDCO 1600 incubator set to 33 C and 5% CO 2 for the duration of the experiment